Lineage markers and cytokine expression were assessed after six hours with a 13-color antibody cocktail described below (Table 1). To generate the data used in this Application Note, cryopreserved human peripheral blood mononuclear cells (PBMC) from healthy donors were thawed and rested overnight for cell function recovery stimulated using Dynabead T-cell Activator anti-CD3/CD28 (ThermoFisher Scientific) in presence of protein transport inhibitor (Brefeldin A). How to use heatmaps to help you identify the phenotype of a specific cluster/metacluster.How FlowSOM can help you in highlighting differences in abundance of specific clusters in your experimental groups.How to leverage a dimensionality reduction algorithm like viSNE for visual comparisons between groups of samples.Plate Loader Options for the CytoFLEX Platform.CytoFLEX Violet-Blue-Red Series Upgrades.Labware for Liquid Handling Instruments.Lysing, Fixative, and Permeabilizating Reagents.RESOURCE Contract Manufacturing Services.

HIAC 9703+ Pharmaceutical Particle Counter.Echo 650 Series Acoustic Liquid Handlers.Biomek NGeniuS Next Generation Library Prep System.Cell Counters, Sizers and Media Analyzers.Air Particle Counters for Cleanroom and Environmental Monitoring.

Heatmap graphpad